EFFECTS OF DIFFERENT CULTURE MEDIA AND PLANT GROWTH REGULATORS ON MICROPROPAGATION OF ʻGISELA 5ʼ CHERRY ROOTSTOCK

Authors: Orsolya BORSAI, Doina CLAPA, Andrei MAGDEA, Monica HÂRȚA, Andreea ANDRECAN, Viorel MITRE

Abstract:

We evaluated the influence of various culture media and plant growth regulators (PGR) upon axillary shoot proliferation rates and shoot lengths obtained in the in vitro multiplication stage in cherry rootstock ‘Gisela 5’. The treatments consisted of the use of three basal media: Driver and Kuniyuki (DKW), Murashige and Skoog (MS), Woody Plant Medium (WPM) as well as three plant growth regulators (PGR) in various concentrations and combinations: N6-benzyladenine (BA at 0.3 and 0.5 mg/l), DL-Dihydrozeatin (DHZ at 1.0 and 2.0 mg/l) and N-benzyl-9-(tetrahydropyranyl)-adenine (BPA at 1.0 mg/l). The results show that the type of basal media and PGRs influenced the quantity as well as the quality of axillary shoot development. In the presence of BA at 0.3 and 0.5 mg/l in DKW media the proliferation rates (PR) were superior to those in MS and WPM media, both at 0.3 mg/l BA (PR = 6.66 ± 0.65) and 0.5 mg/l BA (PR = 8.93 ± 0.86) in the aforementioned DKW treatments. The DKW culture medium supplemented with 1 mg/l indole-3-butyric acid (IBA) provided the highest in vitro rooting percentage (94.74%).

Published: Scientific Papers. Series B, Horticulture. Vol. LXIV, No. 1, 2020 . Varianta tipărită ISSN 2285-5653, CD-ROM ISSN 2285-5661, Varianta Online ISSN 2286-1580, ISSN-L 2285-5653